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vascular endothelial cells vecs  (PromoCell)


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    PromoCell vascular endothelial cells vecs
    Vascular Endothelial Cells Vecs, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vascular endothelial cells vecs/product/PromoCell
    Average 96 stars, based on 235 article reviews
    vascular endothelial cells vecs - by Bioz Stars, 2026-05
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    Insulin and Tumor Necrosis Factor-alpha (TNFα) stimulate increases in VCAM-1 expression in vascular <t>endothelial</t> cells <t>(VEC)</t> . Cells were incubated in growth medium until 80% confluent. Growth medium was removed from cultured cells and replaced with serum-free medium for 24 h. Cells were treated without or with [A] insulin (INS) (10 nM), [B] TNFα (20 ng/mL) or [C] both for designated times and VCAM-1 protein expression was determined via Western blot analysis. Western blots are representative profiles of six assays. [D] Changes in VCAM-1 protein are expressed as the percent of control and represent the mean ± standard error of the mean (SEM) for six independent experiments. *, P < 0.05 vs controls (serum-free medium alone); **, P < 0.05 vs TNFα alone.
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    Insulin and Tumor Necrosis Factor-alpha (TNFα) stimulate increases in VCAM-1 expression in vascular endothelial cells (VEC) . Cells were incubated in growth medium until 80% confluent. Growth medium was removed from cultured cells and replaced with serum-free medium for 24 h. Cells were treated without or with [A] insulin (INS) (10 nM), [B] TNFα (20 ng/mL) or [C] both for designated times and VCAM-1 protein expression was determined via Western blot analysis. Western blots are representative profiles of six assays. [D] Changes in VCAM-1 protein are expressed as the percent of control and represent the mean ± standard error of the mean (SEM) for six independent experiments. *, P < 0.05 vs controls (serum-free medium alone); **, P < 0.05 vs TNFα alone.

    Journal: Journal of Inflammation (London, England)

    Article Title: Insulin augments tumor necrosis factor-alpha stimulated expression of vascular cell adhesion molecule-1 in vascular endothelial cells

    doi: 10.1186/1476-9255-8-34

    Figure Lengend Snippet: Insulin and Tumor Necrosis Factor-alpha (TNFα) stimulate increases in VCAM-1 expression in vascular endothelial cells (VEC) . Cells were incubated in growth medium until 80% confluent. Growth medium was removed from cultured cells and replaced with serum-free medium for 24 h. Cells were treated without or with [A] insulin (INS) (10 nM), [B] TNFα (20 ng/mL) or [C] both for designated times and VCAM-1 protein expression was determined via Western blot analysis. Western blots are representative profiles of six assays. [D] Changes in VCAM-1 protein are expressed as the percent of control and represent the mean ± standard error of the mean (SEM) for six independent experiments. *, P < 0.05 vs controls (serum-free medium alone); **, P < 0.05 vs TNFα alone.

    Article Snippet: Vascular endothelial cells (VEC) were rat aorta vascular cells purchased from ATCC (Manassas, VA) (Cat# CRL 1446) and maintained in culture medium from Gibco/Invitrogen (Carlsbad, CA).

    Techniques: Expressing, Incubation, Cell Culture, Western Blot, Control

    Imaging of temporal progression of pre-vascular networks. Macro (A & C; scalebar = 2 mm) and micro (B & D; scalebar = 100 μm) scale morphology of GFP-endothelial cells (red) in free fibrin hydrogel (A-B) or vascular-3D printed scaffolds (V-3DPSS; C-D) on days 3 (i), 5 (ii), and 7 (iii). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Journal: Acta biomaterialia

    Article Title: 3D printed elastomeric biomaterial mitigates compaction during in vitro vasculogenesis

    doi: 10.1016/j.actbio.2023.09.026

    Figure Lengend Snippet: Imaging of temporal progression of pre-vascular networks. Macro (A & C; scalebar = 2 mm) and micro (B & D; scalebar = 100 μm) scale morphology of GFP-endothelial cells (red) in free fibrin hydrogel (A-B) or vascular-3D printed scaffolds (V-3DPSS; C-D) on days 3 (i), 5 (ii), and 7 (iii). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: Single donor, human umbilical vascular endothelial cells (HU-VECs; Passage 1–6; Cat. No. C2517A; Lonza) or green fluorescent protein-expressing HUVECs (GFP-HUVEC; Passage 1–6; Cat. No. cAP-0001GFP; Angio-Proteomie) were cultured as monolayers in tissue culture treated flasks with media changed every 2 days.

    Techniques: Imaging